The RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis inactivate the Th2 cytokines interleukin-4 and interleukin-5

Abstract

The RgpA-Kgp proteinase-adhesin complexes are a primary virulence factor of Porphyromonas gingivalis,a major pathogen in the development of chronic periodontitis. The RgpA-Kgp complexes have been suggested to bias the immune response to a Th2 phenotype in disease by hydrolysis of Th1 cytokines. Here, we show that the RgpA-Kgp complexes hydrolyze and inactivate interleukin-4 (IL-4) and IL-5 under physiologically relevant conditions. Using the IL-4/IL-5-dependent TF1.8 T-cell line, it was found that at equimolar ratios of cytokine to RgpA-Kgp complexes, IL-4 and IL-5 were inactivated in the culture medium. The inactivation of IL-4 and IL-5 was RgpA-Kgp concentration dependent, as at an enzyme-..