The RgpA-Kgp Proteinase-Adhesin Complexes of Porphyromonas gingivalis Inactivate the Th2 Cytokines Interleukin-4 and Interleukin-5
Vivian Tam, Neil M O'Brien-Simpson, Yu-Yen Chen, Colin J Sanderson, Beverley Kinnear, Eric C Reynolds
INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2009
The RgpA-Kgp proteinase-adhesin complexes are a primary virulence factor of Porphyromonas gingivalis, a major pathogen in the development of chronic periodontitis. The RgpA-Kgp complexes have been suggested to bias the immune response to a Th2 phenotype in disease by hydrolysis of Th1 cytokines. Here, we show that the RgpA-Kgp complexes hydrolyze and inactivate interleukin-4 (IL-4) and IL-5 under physiologically relevant conditions. Using the IL-4/IL-5-dependent TF1.8 T-cell line, it was found that at equimolar ratios of cytokine to RgpA-Kgp complexes, IL-4 and IL-5 were inactivated in the culture medium. The inactivation of IL-4 and IL-5 was RgpA-Kgp concentration dependent, as at an enzyme..View full abstract
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Awarded by Australian National Health and Medical Research Council
This project was supported by the Australian National Health and Medical Research Council (project no. 454475).There are no conflicts of interest.