CHARACTERIZATION OF HISTAMINE PROJECTIONS AND THEIR POTENTIAL CELLULAR TARGETS IN THE MOUSE RETINA
U Greferath, M Kambourakis, C Barth, EL Fletcher, M Murphy
NEUROSCIENCE | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2009
The vertebrate retina receives histaminergic input from the brain via retinopetal axons that originate from perikarya in the posterior hypothalamus. In the nervous system, histamine acts on three G-protein-coupled receptors, histamine receptor (HR) 1, HR2 and HR3. In order to look for potential cellular targets of histamine in the mouse retina, we have examined the retina for the expression of histamine and the presence of these three receptors. Consistent with studies of retina from other vertebrates, histamine was only found in retinopetal axons, which coursed extensively through the ganglion cell and inner plexiform layers. mRNA for all three receptors was expressed in the mouse retina, a..View full abstract
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Awarded by Australian Research Council
We thank Prof. H. Ohtsu Department of Quantum Science and Energy Engineering, Graduate School of Engineering, Tohoku University, Japan, for the supply of the histidine decarboxylase mutant mice and Dr. U. Grunert, National Vision Research Institute of Australia, University of Melbourne, Victoria, Australia, and Dr. C. Anderson, Department of Anatomy and Cell Biology, University of Melbourne, Victoria, Australia for providing antibodies. This work was supported by the Australian Research Council (grant DP0663055). Dr. Ursula Greferath was supported by a Melbourne University Research Fellowship (Career Interruption Scheme).