Journal article

Allelic variance between GRM6 mutants, Grm6(nob3) and Grm6(nob4) results in differences in retinal ganglion cell visual responses

Dennis M Maddox, Kirstan A Vessey, Gary L Yarbrough, Brandon M Invergo, Donald R Cantrell, Samsoon Inayat, Victoria Balannik, Wanda L Hicks, Norman L Hawes, Shannon Byers, Richard S Smith, Ron Hurd, Douglas Howell, Ronald G Gregg, Bo Chang, Juergen K Naggert, John B Troy, Lawrence H Pinto, Patsy M Nishina, Maureen A McCall

JOURNAL OF PHYSIOLOGY-LONDON | WILEY-BLACKWELL | Published : 2008

Abstract

An electroretinogram (ERG) screen identified a mouse with a normal a-wave but lacking a b-wave, and as such it was designated no b-wave3 (nob3). The nob3 phenotype mapped to chromosome 11 in a region containing the metabotropic glutamate receptor 6 gene (Grm6). Sequence analyses of cDNA identified a splicing error in Grm6, introducing an insertion and an early stop codon into the mRNA of affected mice (designated Grm6(nob3)). Immunohistochemistry of the Grm6(nob3) retina showed that GRM6 was absent. The ERG and visual behaviour abnormalities of Grm6(nob3) mice are similar to Grm6(nob4) animals, and similar deficits were seen in compound heterozygotes (Grm6(nob4/nob3)), indicating that Grm6(n..

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University of Melbourne Researchers

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Awarded by National Eye Institute


Awarded by NIH Cooperative Research Agreement


Awarded by The Jackson Laboratory


Awarded by NATIONAL CANCER INSTITUTE


Awarded by NATIONAL EYE INSTITUTE


Awarded by NATIONAL INSTITUTE OF MENTAL HEALTH


Funding Acknowledgements

The authors would like to thank Ms. Elaine Gifford, Ms. Jeanie Hansen, Ms. Karen Moore, Ms. Jennifer Torrance and Ms. Barbra Mortimer for excellent technical assistance. This work was supported by National Eye Institute grants: EY11996 (PMN), EY016501 (PMN), EY014701, (MAMc), EY012354, (RGG), EY007758 (BC), EY06669 (JBT), EY016313 (DMM), a National Defense Science & Training Fellowship (DRC), NIH Cooperative Research Agreement U01MH61915 (LHP), an Institutional Core Grant to The Jackson Laboratory (CA34196), and R24EY015636 and an unrestricted from Research to Prevent Blindness, Inc., NY to the Department of Ophthalmology & Visual Sciences, University of Louisville.