Journal article

Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging

Koon Ho Wong, Richard B Todd, Berl R Oakley, C Elizabeth Oakley, Michael J Hynes, Meryl A Davis

FUNGAL GENETICS AND BIOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2008

Abstract

Sumoylation, the reversible covalent attachment of small ubiquitin-like modifier (SUMO) peptides has emerged as an important regulator of target protein function. In Saccharomyces cerevisiae, but not in Schizosaccharyomes pombe, deletion of the gene encoding SUMO peptides is lethal. We have characterized the SUMO-encoding gene, sumO, in the filamentous fungus Aspergillus nidulans. The sumO gene was deleted in a diploid and sumODelta haploids were recovered. The mutant was viable but exhibited impaired growth, reduced conidiation and self-sterility. Overexpression of epitope-tagged SumO peptides revealed multiple sumoylation targets in A. nidulans and SumO overexpression resulted in greatly i..

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