Journal article

Expression of gastrin precursors by CD133-positive colorectal cancer cells is crucial for tumour growth

Audrey Ferrand, Mauro S Sandrin, Arthur Shulkes, Graham S Baldwin

Biochimica et Biophysica Acta - Molecular Cell Research | ELSEVIER | Published : 2009

Abstract

Precursors of the hormone gastrin, progastrin and glycine-extended gastrin (G-gly), have been detected in colorectal polyps and tumours, and in the blood of patients with colorectal cancer (CRC), while their expression is lower in healthy subjects. The surface glycoproteins CD133 and CD44 have been identified as possible markers for CRC stem cells. Our aims were to investigate whether progastrin and G-gly are expressed by CD133-positive cells in human CRC tissues and in the human CRC cell line DLD-1, and to determine whether this expression is biologically relevant. The great majority of the cells expressing CD133 also expressed gastrin precursors in both DLD-1 cells, which retain a stem cel..

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Grants

Awarded by National Institutes of Health


Awarded by National Health and Medical Research Council of Australia


Awarded by Medical Research and Technology in Victoria


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

We thank Natalie Dodge and Russel Hodgson for their help with the FACS experiments. This work is supported by grant 5 R01 GM065926-06 from the National Institutes of Health (to GB, AS), grants 400062 (to GB, AS), 208926 (to GB) and 350235 (to AS) from the National Health and Medical Research Council of Australia, grant CT8917 from Medical Research and Technology in Victoria which is managed by ANZ Trustees (to AF), and a grant from the Austin Medical Research Foundation (to AF).