Relationship between Human Immunodeficiency Virus Type 1 Coinfection, Anemia, and Levels and Function of Antibodies to Variant Surface Antigens in Pregnancy-Associated Malaria
Anthony Jaworowski, Liselle A Fernandes, Francisca Yosaatmadja, Gaoqian Feng, Victor Mwapasa, Malcolm E Molyneux, Steven R Meshnick, Jenny Lewis, Stephen J Rogerson
CLINICAL AND VACCINE IMMUNOLOGY | AMER SOC MICROBIOLOGY | Published : 2009
Human immunodeficiency virus type 1 (HIV-1) coinfection decreases antibodies to variant surface antigens implicated in pregnancy-associated malaria (VSA-PAM) caused by Plasmodium falciparum. The effect of HIV-1 on antibody functions that may protect mothers from pregnancy-associated malaria is unknown. Sera from multigravid pregnant women with malaria and HIV-1 coinfection (n=58) or malaria alone (n=29) and from HIV-1-infected (n=102) or -uninfected (n=54) multigravidae without malaria were analyzed for anti-VSA-PAM antibodies by flow cytometry, the ability to inhibit adhesion to chondroitin sulfate A, or to opsonize CS2-infected erythrocytes for phagocytosis by THP-1 cells. In women with ma..View full abstract
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We thank Terrie Taylor for providing the male plasma samples, Aphrodite Caragounis for technical assistance, James Blaszak for early assistance with establishing opsonic activity assays, Tim Spelman for statistical advice, and James Beeson for advice with adhesion inhibition assays. We thank the pregnant women who kindly participated in this study, who were recruited as part of NIH RO1 award number AI 49084.This work was supported by NHMRC project grant 400090 to S.J.R. and A.J.