Rapid Degranulation of NK Cells following Activation by HIV-Specific Antibodies
Amy W Chung, Erik Rollman, Rob J Center, Stephen J Kent, Ivan Stratov
JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2009
Ab-dependent cellular cytotoxicity (ADCC) Abs stimulate NK cell effector functions and play a role in protecting from and controlling viral infections. We characterized ADCC Abs in a cross-sectional cohort of 80 HIV-infected subjects not on antiretroviral therapy. We analyzed ADCC response by killing fluorescently labeled target cells, as well as expression of IFN-gamma and the degranulation marker CD107a from activated NK cells as measured by a novel intracellular cytokine assay. HIV-specific ADCC directed toward Envelope proteins were present in the majority of 80 untreated HIV-infected individuals measured by killing function. Similarly, most subjects had HIV-specific Abs that mediated de..View full abstract
Awarded by Australian National Health and Medical Research Council
Awarded by Swedish Research Council
Supported by Australian National Health and Medical Research Council awards 299907, 359233, and 251654, and Swedish Research Council Fellowship 2005-2527.