Journal article
Comparative transcriptomic analysis of Porphyromonas gingivalis biofilm and planktonic cells
AW Lo, CA Seers, JD Boyce, SG Dashper, N Slakeski, JP Lissel, EC Reynolds
BMC Microbiology | Published : 2009
Abstract
Background. Porphyromonas gingivalis in subgingival dental plaque, as part of a mature biofilm, has been strongly implicated in the onset and progression of chronic periodontitis. In this study using DNA microarray we compared the global gene expression of a P. gingivalis biofilm with that of its planktonic counterpart grown in the same continuous culture. Results. Approximately 18% (377 genes, at 1.5 fold or more, P-value < 0.01) of the P. gingivalis genome was differentially expressed when the bacterium was grown as a biofilm. Genes that were down-regulated in biofilm cells, relative to planktonic cells, included those involved in cell envelope biogenesis, DNA replication, energy productio..
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Awarded by National Institutes of Health
Funding Acknowledgements
This work was supported by the Australian National Health and Medical Research Council (Project Grant No. 300006) and Australian Government's Cooperative Research Centres program, through the Cooperative Research Centre for Oral Health Science. Microarray slides were kindly provided by TIGR and NIDCR. We also thank Rebecca Fitzgerald for helpful discussions on real time reverse transcription-PCR analysis. The following material was obtained through NIAID's Pathogen Functional genomics Resource Center, managed and funded by Division of Microbiology and Infectious Diseases, NIAID, NIH, DHHS and operated by the J. Craig Center Institute.