Journal article

Identification of glucocorticoid-regulated genes that control cell proliferation during murine respiratory development

Anthony D Bird, Kheng H Tan, P Fredrik Olsson, Malgorzata Zieba, Sharon J Flecknoe, Douglas R Liddicoat, Richard Mollard, Stuart B Hooper, Timothy J Cole



Glucocorticoids play a vital role in fetal respiratory development and act via the intracellular glucocorticoid receptor (GR) to regulate transcription of key target genes. GR-null mice die at birth due to respiratory dysfunction associated with hypercellularity and atelectasis. To identify events associated with this lung phenotype we examined perinatal cellular proliferation rates and apoptotic indices. We demonstrate that compared to wild-type controls, day 18.5 postcoitum (p.c.) GR-null mouse lungs display significantly increased cell proliferation rates (1.8-fold P < 0.05) and no change in apoptosis. To examine underlying molecular mechanisms, we compared whole genome expression profile..

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University of Melbourne Researchers