Journal article

Division-linked differentiation can account for CD8( ) T-cell phenotype in vivo

Timothy E Schlub, Vanessa Venturi, Katherine Kedzierska, Cameron Wellard, Peter C Doherty, Stephen J Turner, Ruy M Ribeiro, Philip D Hodgkin, Miles P Davenport



The CD8(+) T-cell response to infection involves a large initial expansion in the numbers of responding cells, accompanied by differentiation of these cells. Expression of the adhesion molecule CD62L is high on naïve cells and rapidly downregulated on the surface of the majority (approximately 90%) of cells during the 'effector' phase of acute infection. Adoptive transfer studies have been used to study differentiation in this system; however, relatively little work has investigated the phenotype of cells in the endogenous repertoire. We demonstrate that the extent of CD62L down-regulation is positively correlated with clone size in vivo, consistent with division-linked differentiation of re..

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Funding Acknowledgements

This work was supported by the James S. McDonnell Foundation 21st Century Research Award/Studying Complex Systems, the Australian National Health and Medical Research Council (NHMRC) and a Bumet award of the NHMRC and Science, Technology, and Innovation funds from the Government of Victoria, Australia (to P.C.D.). M.P.D. is a Sylvia and Charles Viertel senior medical research fellow. K.K. is an NHMRC RD Wright fellow and S.J.T. is a Pfizer senior research fellow.