Journal article

Buffy, a Drosophila Bcl-2 protein, has anti-apoptotic and cell cycle inhibitory functions

L Quinn, M Coombe, K Mills, T Daish, P Colussi, S Kumar, H Richardson

EMBO JOURNAL | WILEY | Published : 2003

Abstract

Bcl-2 family proteins are key regulators of apoptosis. Both pro-apoptotic and anti-apoptotic members of this family are found in mammalian cells, but only the pro-apoptotic protein Debcl has been characterized in Drosophila: Here we report that Buffy, the second Drosophila Bcl-2-like protein, is a pro-survival protein. Ablation of Buffy by RNA interference leads to ectopic apoptosis, whereas overexpression of buffy results in the inhibition of developmental programmed cell death and gamma irradiation-induced apoptosis. Buffy interacts genetically and physically with Debcl to suppress Debcl-induced cell death. Genetic interactions suggest that Buffy acts downstream of Rpr, Grim and Hid, and u..

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