Journal article

Identification of ARAP3, a novel PI3K effector regulating both Arf and Rho GTPases, by selective capture on phosphoinositide affinity matrices

S Krugmann, KE Anderson, SH Ridley, N Risso, A McGregor, J Coadwell, K Davidson, A Eguinoa, CD Ellson, P Lipp, M Manifava, N Ktistakis, G Painter, JW Thuring, MA Cooper, ZY Lim, AB Holmes, SK Dove, RH Michell, A Grewal Show all

MOLECULAR CELL | CELL PRESS | Published : 2002


We show that matrices carrying the tethered homologs of natural phosphoinositides can be used to capture and display multiple phosphoinositide binding proteins in cell and tissue extracts. We present the mass spectrometric identification of over 20 proteins isolated by this method, mostly from leukocyte extracts: they include known and novel proteins with established phosphoinositide binding domains and also known proteins with surprising and unusual phosphoinositide binding properties. One of the novel PtdIns(3,4,5)P3 binding proteins, ARAP3, has an unusual domain structure, including five predicted PH domains. We show that it is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6 GAP ..

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University of Melbourne Researchers