Journal article
Synthesis and biological evaluation of a PtdIns(4,5)P2and a phosphatidic acid affinity matrix
ZY Lim, JW Thuring, AB Holmes, M Manifava, NT Ktistakis
Journal of the Chemical Society Perkin Transactions 1 | ROYAL SOC CHEMISTRY | Published : 2002
DOI: 10.1039/b200585a
Abstract
Analogues of dipalmitoyl phosphatidic acid (PA), dilauroyl PA and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] were synthesised and immobilised onto a solid support, Affi-Gel 10. Using them as affinity matrices, a number of known proteins as well as a set of novel proteins were found to bind specifically to PA. © 2002 The Royal Society of Chemistry.