Synthesis and biological evaluation of a PtdIns(4,5)P-2 and a phosphatidic acid affinity matrix
ZY Lim, JW Thuring, AB Holmes, M Manifava, NT Ktistakis
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | ROYAL SOC CHEMISTRY | Published : 2002
Analogues of dipalmitoyl phosphatidic acid (PA), dilauroyl PA and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] were synthesised and immobilised onto a solid support, Affi-Gel 10. Using them as affinity matrices, a number of known proteins as well as a set of novel proteins were found to bind specifically to PA. © 2002 The Royal Society of Chemistry.