Major proteins and antigens of Treponema denticola
Paul D Veith, Stuart G Dashper, Neil M O'Brien-Simpson, Rita A Paolini, Rebecca Orth, Katrina A Walsh, Eric C Reynolds
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | ELSEVIER | Published : 2009
Treponema denticola is a Gram-negative, motile, asaccharolytic, anaerobic spirochaete which along with Porphyromonas gingivalis and Tannerella forsythia has been shown to form a bacterial consortium called the Red Complex that is strongly associated with the clinical progression of chronic periodontitis. T. denticola was grown in continuous culture in a complex medium with a mean generation time of 15.75 h. Samples from two different membrane-enriched preparations and a cytoplasm-enriched preparation were separated by two-dimensional gel electrophoresis and the proteins identified by MALDI-TOF/TOF mass spectrometry. In total, 219 non-redundant proteins were identified including numerous viru..View full abstract
This research was funded by a National Health and Medical Research Council Australia project grant.