A barley cellulose synthase-like CSLH gene mediates (1,3;1,4)-beta-D-glucan synthesis in transgenic Arabidopsis
Monika S Doblin, Filomena A Pettolino, Sarah M Wilson, Rebecca Campbell, Rachel A Burton, Geoffrey B Fincher, Ed Newbigin, Antony Bacic
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2009
The walls of grasses and related members of the Poales are characterized by the presence of the polysaccharide (1,3, 1,4)-beta-D-glucan (beta-glucan). To date, only members of the grass-specific cellulose synthase-like F (CSLF) gene family have been implicated in its synthesis. Assuming that other grass-specific CSL genes also might encode synthases for this polysaccharide, we cloned HvCSLH1, a CSLH gene from barley (Hordeum vulgare L.), and expressed an epitope-tagged version of the cDNA in Arabidopsis, a species with no CSLH genes and no beta-glucan in its walls. Transgenic Arabidopsis lines that had detectable amounts of the epitope-tagged HvCSLH1 protein accumulated beta-glucan in their ..View full abstract
We thank Margie Pallotta and Neil Shirley for their assistance with gene mapping and qPCR, respectively, Dr. Anne Medhurst for her assistance during the early stages of this work, and Drs. Tsuyoshi Nakagawa (Shimane University, Japan) and Maria Hrmova for providing the pGWB vectors and beta-glucan hydrolase for immuno-EM work, respectively. This work was supported by funding from the Grains Research and Development Corporation, the Australian Research Council, and the Commonwealth Scientific and Research Organization Flagship Collaborative Research Program, provided to the High Fibre Grains Cluster via the Food Futures Flagship.