Restoration of full-length adenomatous polyposis coli (APC) protein in a colon cancer cell line enhances cell adhesion

Abstract

The APC tumour suppressor gene is mutated in most colon cancers. A major role of APC is the downregulation of the β-catenin/T-cell factor (Tcf)/lymphoid enhancer factor (LEF) signalling pathway; however, there are also suggestions that it plays a role in the organization of the cytoskeleton, and in cell adhesion and migration. For the first time, we have achieved stable expression of wild-type APC in SW480 colon cancer cells, which normally express a truncated form of APC. The ectopically expressed APC is functional, and results in the translocation of β-catenin from the nucleus and cytoplasm to the cell periphery, and reduces β-catenin/Tcf/LEF transcriptional signalling. E-cadherin is also ..