Journal article

Role of activator protein 1, nuclear factor-kappa B, and nuclear factor of activated T cells in IgE receptor-mediated cytokine expression in mature human mast cells

A Lorentz, I Klopp, T Gebhardt, MP Manns, SC Bischoff

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | MOSBY, INC | Published : 2003

Abstract

BACKGROUND: On activation by cross-linking the high-affinity IgE receptor (FcepsilonRI), expression of TNF-alpha, IL-3, IL-5, and IL-13 is induced in human intestinal mast cells. OBJECTIVE: We sought to examine, for the first time, FcepsilonRI signaling in mature human mast cells. METHODS: Mast cells were isolated from intestinal tissue and cultured in the presence of stem cell factor. The cells were treated with specific inhibitors before stimulation by means of FcepsilonRI cross-linking. Cytokine mRNA expression was analyzed by means of RT-PCR, and activation of signaling molecules was determined by means of immunocytochemistry, RT-PCR, Western blotting, and protein kinase C (PKC) assay. R..

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