Journal article

Impaired complement-mediated phagocytosis by HIV type-1-infected human monocyte-derived macrophages involves a cAMP-dependent mechanism

Rula Azzam, Katherine Kedzierska, Edwin Leeansyah, Hiutat Chan, Daniel Doischer, Paul R Gorry, Anthony L Cunningham, Suzanne M Crowe, Anthony Jaworowski

AIDS RESEARCH AND HUMAN RETROVIRUSES | MARY ANN LIEBERT, INC | Published : 2006

Abstract

HIV-1 infection of cells of macrophage lineage impairs a number of effector functions performed by these cells, including phagocytosis of opsonized pathogens. In this study we investigate the effects of HIV-1 on the mechanism of complement (C')-mediated phagocytosis by human monocyte-derived macrophages (MDM). Using C'-opsonized sheep red blood cells (sRBC) as targets, we demonstrate that phagocytosis is inhibited by HIV-1 infection in vitro. Inhibition is not due to downregulation of surface C' receptors (R) or altered binding of C'-opsonized targets to HIV-1-infected MDM, suggesting a postreceptor-mediated mechanism of suppression. Having shown that increased levels of intracellular cAMP i..

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