Journal article

Metabolic profiling of transgenic wheat over-expressing the high-molecular-weight Dx5 glutenin subunit

Boryana S Stamova, Ute Roessner, Suganthi Suren, Debbie Laudencia-Chingcuanco, Antony Bacic, Diane M Beckles

METABOLOMICS | SPRINGER | Published : 2009

Abstract

The primary aim of this work was to evaluate potential changes in the metabolic network of transgenic wheat grain over-expressing the high-molecular-weight (HMW) glutenin Dx5-subunit gene. GC-MS and multivariate analyses were used to compare the metabolite profiles of developing caryopses of two independently transformed lines over-expressing Dx5 and another two independently transformed lines expressing only the selectable-marker gene (controls). Developing grain at 7, 14 and 21 Days Post-Anthesis (DPA) was studied to observe differences in metabolically active tissues. There was no distinction between the Dx5 transformants and the controls by principal component analysis (PCA) suggesting t..

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Grants

Awarded by USDA-ARS


Awarded by National Science Foundation


Funding Acknowledgements

We thank Dr. Ann Blechl for the generous gift of the transgenic seeds and information on the lines. We are indebted to Dr. Ron Haff, USDA-ARS Albany, for help with SAS Software and to Nick Petkov and Dobromir Tzankov for technical assistance. We thank Drs. Belinda Martineau and Olin Anderson for comments on the manuscript. This work was supported by grants to the Australian Centre for Plant Functional Genomics from the Grains Research and Development Corporation, the Australian Research Council, the South Australian Government, the University of Adelaide and the University of Melbourne (SS, UR, AB); and to Metabolomics Australia provided by the Australian Government through the National Collaborative Research Infrastructure Strategy (UR, AB), USDA-ARS CRIS Project 5325-21000-011 (DLC, BS) and National Science Foundation Grant: NSF-MCB-0620001 (DMB).