Journal article

Effect of Probiotic strains on interleukin 8 production by HT29/19A cells

KM Lammers, U Helwig, E Swennen, F Rizzello, A Venturi, E Caramelli, MA Kamm, P Brigidi, P Gionchetti, M Campieri

AMERICAN JOURNAL OF GASTROENTEROLOGY | NATURE PUBLISHING GROUP | Published : 2002

Abstract

OBJECTIVES: Promising results from clinical studies on the effect of probiotics as maintenance therapy in inflammatory bowel disease and in the prevention of onset of pouchitis ask for studies to unravel the still poorly understood mechanism of action of probiotics. METHODS: To evaluate whether the probiotic bacteria that were used in the clinical studies (VSL#3, Escherichia coli Nissle 1917, and Lactobacillus GG) are able to induce chemokine production in epithelial cells, HT29/19A monolayers were incubated with cell debris and cell extract fractions of single strains of the probiotic bacteria in doses ranging from 10(3) to 10(9) colony-forming units/ml for 32 h. Supernatants were measured ..

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