Journal article

Up-regulation of collagen and tissue inhibitors of matrix metalloproteinase in colonic diverticular disease

T Mimura, AC Bateman, RL Lee, PA Johnson, PJ McDonald, IC Talbot, MA Kamm, TT MacDonald, SLF Pender

DISEASES OF THE COLON & RECTUM | LIPPINCOTT WILLIAMS & WILKINS | Published : 2004

Abstract

PURPOSE: Thickening of the muscularis propria is a key pathologic feature of colonic diverticulosis but its cause is unknown. This study was designed to investigate the role of collagens, matrix metalloproteinases, and tissue inhibitor of metalloproteinases in colonic diverticulosis. METHODS: Collagen content was determined by Sircol Collagen Assay and standard van Gieson staining. Messenger-RNA expression for matrix metalloproteinases and tissue inhibitor of metalloproteinase was analyzed by quantitative competitive reverse transcription polymerase chain reaction. Immunohistochemical staining was performed to localize tissue inhibitor of metalloproteinases in sections. RESULTS: In mucosa an..

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