Journal article
The generation and properties of human macrophage populations from hemopoietic stem cells
KJ Way, H Dinh, MR Keene, KE White, FIL Clanchy, P Lusby, J Roiniotis, AD Cook, AI Cassady, DJ Curtis, JA Hamilton
Journal of Leukocyte Biology | WILEY | Published : 2009
DOI: 10.1189/jlb.1108689
Abstract
Information about the development and function of human macrophage lineage populations, such as osteoclasts, is limited because of the lack of defined in vitro systems for their largescale generation. Two M-CSF-containing cytokine cocktails were found under serum-free conditions to expand dramatically and to differentiate over time human CD34+ hemopoietic stem cells into nonadherent and adherent macrophage populations. These populations exhibited increasing degrees of maturity over a 3-week period characterized by morphology, surface marker expression (CD11b, CD86, CD64, CD14, and c-Fms), phagocytic function, and gene-expression profiling using quantitative PCR and microarray analysis (princ..
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Funding Acknowledgements
This work is supported by a grant and Senior Principal Research Fellowship ( to J. A. H) from the National Health and Medical Research Council of Australia and by the Cooperative Research Centre for Chronic Inflammatory Diseases. We thank C. Dowsing, G. Bueno, J. Hicks, and J. Szer ( Royal Melbourne Hospital) for procuring the leukapheresis product and D. Haylock and P. Simmons for helpful discussions.