Journal article
Low dose metal particles can induce monocyte/macrophage survival
DC Lacey, B De Kok, FI Clanchy, MJ Bailey, K Speed, D Haynes, SE Graves, JA Hamilton
Journal of Orthopaedic Research | JOHN WILEY & SONS INC | Published : 2009
DOI: 10.1002/jor.20914
Abstract
Aseptic loosening results in pain, loss of function, and ultimately prosthetic joint failure and revision surgery. The generation of wear particles from the prosthesis is a major factor in local osteolysis. We investigated the effects of such wear particles on the survival of monocytes and macrophages, populations implicated in wear particle-driven pathology. Particles from titanium aluminum vanadium (TiAlV) and cobalt chromium (CoCr) alloys were generated in-house and were equivalent in size (0.5-3 μm) to those seen in patients.Human CD14+ monocytes and murine bone marrow-derived macrophages (BMM) were treated with TiAlV and CoCr particles in vitro, and cell survival was assayed. Both parti..
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Funding Acknowledgements
We acknowledge Ms. L. Crouch for her technical assistance in particle preparation and in performing a number of experiments. This work was supported in part by the Cooperative Research Centre for Chronic Inflammatory Diseases and grants from the National Health and Medical Research Council to J. A. H. D. C. L. is supported by the AFA-ARA Heald Fellowship from the Arthritis Foundation of Australia.