Journal article
Characterization of Chlamydiaceae species using PCR and high resolution melt curve analysis of the 16S rRNA gene
T Robertson, S Bibby, D O'Rourke, T Belfiore, H Lambie, AH Noormohammadi
Journal of Applied Microbiology | WILEY | Published : 2009
Abstract
Aim: To design a rapid diagnostic test to differentiate species belonging to the family Chlamydiaceae. Methods and Results: Five oligonucleotide sets each targeting various conserved regions of the genome of six species (Chlamydia muridarum, C. suis, C. trachomatis, Chlamydophila felis, Cp. pneumoniae and Cp. psittaci) belonging to the family Chlamydiaceae were tested for their suitability for polymerase chain reaction (PCR) and high resolution melt (HRM) curve analysis to differentiate Chlamydiaceae species. Three of the oligonucleotide sets were able to detect all six reference species used in this study, but only one set (16SG) could clearly differentiate between them by HRM curve analysi..
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Funding Acknowledgements
This study was supported by the Australian Rural Industries Research and Development Corporation (RIRDC) Chicken Meat Branch and the Australian Poultry Cooperative Research Council (CRC). The authors also thank Professor Daisy Vanrompay, University of Ghent, Belgium, for providing the Cp. psittaci genomic DNA used in this study.