Journal article

Characterization of Chlamydiaceae species using PCR and high resolution melt curve analysis of the 16S rRNA gene

T Robertson, S Bibby, D O'Rourke, T Belfiore, H Lambie, AH Noormohammadi

Journal of Applied Microbiology | WILEY | Published : 2009

Abstract

AIM: To design a rapid diagnostic test to differentiate species belonging to the family Chlamydiaceae. METHODS AND RESULTS: Five oligonucleotide sets each targeting various conserved regions of the genome of six species (Chlamydia muridarum, C. suis, C. trachomatis, Chlamydophila felis, Cp. pneumoniae and Cp. psittaci) belonging to the family Chlamydiaceae were tested for their suitability for polymerase chain reaction (PCR) and high resolution melt (HRM) curve analysis to differentiate Chlamydiaceae species. Three of the oligonucleotide sets were able to detect all six reference species used in this study, but only one set (16SG) could clearly differentiate between them by HRM curve analysi..

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