Journal article
Proinflammatory cytokines inhibit osteogenic differentiation from stem cells: implications for bone repair during inflammation
DC Lacey, PJ Simmons, SE Graves, JA Hamilton
Osteoarthritis and Cartilage | ELSEVIER SCI LTD | Published : 2009
Abstract
Objective: The effects of inflammation on bone development from mesenchymal stem cells (MSC) are unclear due to the difficulty in isolating MSC. The aim of this study was to develop a MSC isolation method and to determine the in vitro effects of interleukin-1β (IL-1β) and tumor necrosis factor α (TNFα) on their osteogenic differentiation. Methods: Murine MSC were isolated from the limbs of C57/Bl6 mice through collagenase digestion of bone and enriched as the Stem cell antigen (Sca-1)+ CD31- CD45- population, using lineage immunodepletion, followed by fluorescence-activated cell sorting (FACS). They were differentiated along the osteoblast linage in the presence or absence of IL-1β and TNFα...
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Funding Acknowledgements
This work was supported in part by the Cooperative Research Centre (CRC) for Chronic Inflammatory Diseases and grants from the National Health and Medical Research Council to JAH. DCL is supported by the AFA-ARA Heald Fellowship from the Arthritis Foundation of Australia.Supported by the Cooperative Research Centre (CRC) for Chronic Inflammatory Diseases. Prof John A. Hamilton's work was also supported by grants from the National Health and Medical Research Council of Australia. Dr Derek C. Lacey was supported by an AFA-ARA Heald Fellowship from the Arthritis foundation of Australia.