Hyphal cell walls from the plant pathogen Rhynchosporium secalis contain (1,3/1,6)-beta-d-glucans, galacto- and rhamnomannans, (1,3;1,4)-beta-d-glucans and chitin
Filomena Pettolino, Izumi Sasaki, Alisa Turbic, Sarah M Wilson, Antony Bacic, Maria Hrmova, Geoffrey B Fincher
FEBS Journal | WILEY | Published : 2009
A procedure has been developed for the isolation of cell walls from the hyphae of the causal agent for barley leaf scald, Rhynchosporium secalis (Oudem) J.J. Davis. Based primarily on monosaccharide linkage analysis, but also on the limited use of linkage-specific glucan hydrolases and solvent fractionation, the walls consist predominantly of (1,3/1,6)-beta-D-glucans, (1,3;1,4)-beta-D-glucans, galactomannans of (1,2;1,6)-Manp residues and (1,5)-galactofuranosyl [(1,5)-Galf] side chains, rhamnomannans of (1,6)-Manp residues and rhamnopyranosyl [(1,2)-Rhap] side chains, and chitin; the walls also contain approximately 23% (w/w) protein. Electron microscopy shows the presence of distinct inner ..View full abstract
The work was supported by grants from the Australian Research Council (to GBF and MH) and the Grains Research and Development Corporation (to AB and GBF). We thank Hugh Wallwork and Felicity Keiper for providing the fungal isolate and for helpful advice.