Hyphal cell walls from the plant pathogen Rhynchosporium secalis contain (1,3/1,6)-β-D-glucans, galacto- and rhamnomannans, (1,3;1,4)-β-D- glucans and chitin

Abstract

A procedure has been developed for the isolation of cell walls from the hyphae of the causal agent for barley leaf scald, Rhynchosporium secalis (Oudem) J.J. Davis. Based primarily on monosaccharide linkage analysis, but also on the limited use of linkage-specific glucan hydrolases and solvent fractionation, the walls consist predominantly of (1,3/1,6)-β-d-glucans, (1,3;1,4)-β-d-glucans, galactomannans of (1,2;1,6)-Manp residues and (1,5)-galactofuranosyl [(1,5)-Galf] side chains, rhamnomannans of (1,6)-Manp residues and rhamnopyranosyl [(1,2)-Rhap] side chains, and chitin; the walls also contain ≈23% (w/w) protein. Electron microscopy shows the presence of distinct inner and outer wall laye..