Journal article

The conserved metalloprotease invadolysin localizes to the surface of lipid droplets

Neville Cobbe, Kathryn M Marshall, Shubha Gururaja Rao, Ching-Wen Chang, Francesca Di Cara, Edward Duca, Sharron Vass, Adam Kassan, Margarete MS Heck



Invadolysin is a metalloprotease conserved in many different organisms, previously shown to be essential in Drosophila with roles in cell division and cell migration. The gene seems to be ubiquitously expressed and four distinct splice variants have been identified in human cells but not in most other species examined. Immunofluorescent detection of human invadolysin in cultured cells reveals the protein to be associated with the surface of lipid droplets. By means of subcellular fractionation, we have independently confirmed the association of invadolysin with lipid droplets. We thus identify invadolysin as the first metalloprotease located on these dynamic organelles. In addition, analysis..

View full abstract

University of Melbourne Researchers


Awarded by EU

Funding Acknowledgements

The authors would like to express their gratitude to Bill Earnshaw and Mike Cousin for interesting and relevant discussions, as well as to Paddy Hadoke, Damien Hudson and Heck lab members for feedback on the manuscript. We are grateful to Grant Sellar, Laura Machesky, David Melton, Bill Earnshaw, Jim Ross and Adriano Rossi for cell lines and cultures. Albert Pol (Barcelona) was extremely helpful in advising on the isolation of lipid droplets from cultured cells. Walid Maalouf and Sari Pennings provided assistance with OptiGrid Structured Illumination Microscopy. This work was supported by a Wellcome Trust University Award to M. M. S. H., a postdoctoral fellowship from Medical Research Scotland to K. M. M., and a Darwin Trust PhD studentship to S. G. R. Work with orang-utan cell lines was supported by EUPRIM-Net under the EU contract RII3-026155 of the 6th Framework Programme. Deposited in PMC for release after 6 months.