Characterization of PiB Binding to White Matter in Alzheimer Disease and Other Dementias
Michelle T Fodero-Tavoletti, Christopher C Rowe, Catriona A McLean, Laura Leone, Qiao-Xin Li, Colin L Masters, Roberto Cappai, Victor L Villemagne
JOURNAL OF NUCLEAR MEDICINE | SOC NUCLEAR MEDICINE INC | Published : 2009
UNLABELLED: 11C-Pittsburgh Compound B (11C-PiB) PET has demonstrated significantly higher PiB retention in the gray matter of Alzheimer disease (AD) patients than in healthy controls (HCs). PiB is similarly retained within the white matter of HC and AD brains. Although the specificity of PiB for Abeta plaques in gray matter has been well described, the nature of PiB binding to white matter remains unclear. In this study, we characterized the binding of PiB to human white matter homogenates. METHODS: In vitro binding studies were conducted using 3H-PiB (0.1-500 nM) and white matter brain homogenates (100 microg) from 3 AD patients and 3 HCs. Nonspecific binding was determined using PiB (1 mic..View full abstract
We thank Henri Tochon-Danguy, Graeme O'Keefe, Uwe Ackermann, Rachel Mulligan, Jessica Sagona, Kunthi Pathmaraj, Tim Saunder, Jason Bradley, and Gareth Jones for their crucial role during radiochemical synthesis, PET examinations, and image processing; Tiffany Cowie for ApoE genotyping; Katrina Laughton for ELISA processing; and Fairlie Hinton and Geoff Pavey from the National Neural Tissue Resource Centre for sourcing and preparing the human brain tissue. This work was Supported in part by the National Health and Medical Research Council of Australia, the Austin Hospital Medical Research Foundation, and Neurosciences Victoria.