Journal article
Characterization of Drosophila melanogaster cytochrome P450 genes
H Chung, T Sztal, S Pasricha, M Sridhar, P Batterham, PJ Daborn
Proceedings of the National Academy of Sciences of the United States of America | Published : 2009
Abstract
Cytochrome P450s form a large and diverse family of hemecontaining proteins capable of carrying out many different enzymatic reactions. In both mammals and plants, some P450s are known to carry out reactions essential for processes such as hormone synthesis, while other P450s are involved in the detoxification of environmental compounds. In general, functions of insect P450s are less well understood. We characterized Drosophila melanogaster P450 expression patterns in embryos and 2 stages of third instar larvae. We identified numerous P450s expressed in the fat body, Malpighian (renal) tubules, and in distinct regions of the midgut, consistent with hypothesized roles in detoxification proces..
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Funding Acknowledgements
The Australian Drosophila Biomedical Research Support Facility is acknowledged for providing Drosophila services. The Vienna Drosophila RNAi Center and Bloomington Drosophila Stock Center are acknowledged for providing Drosophila stocks. We thank Charles Robin, Robert Good, and Lydia Gramzow (University of Melbourne) for valuable discussion and sharing unpublished data, Alex Andrianopoulos (University of Melbourne) for valuable discussion, and Gary Hime (University of Melbourne) for help with microscopy. This work was supported by the Australian Research Council through its funding of the Centre of Environmental Stress and Adaptation Research and an Australian Research Fellowship (to P. J. D.).