Journal article
The lewis-Y carbohydrate antigen is expressed by many human tumors and can serve as a target for genetically redirected T cells despite the presence of soluble antigen in serum
JA Westwood, WK Murray, M Trivett, NM Haynes, B Solomon, L Mileshkin, D Ball, M Michael, A Burman, P Mayura-Guru, JA Trapani, S Peinert, D Hönemann, H Miles Prince, AM Scott, MJ Smyth, PK Darcy, MH Kershaw
Journal of Immunotherapy | LIPPINCOTT WILLIAMS & WILKINS | Published : 2009
Abstract
In this study we aimed to determine the suitability of the Lewis-Y carbohydrate antigen as a target for immunotherapy using genetically redirected T cells. Using the 3S193 monoclonal antibody and immunohistochemistry, Lewis-Y was found to be expressed on a range of tumors including 42% squamous cell lung carcinoma, 80% lung adenocarcinoma, 25% ovarian carcinoma, and 25% colorectal adenocarcinoma. Expression levels varied from low to intense on between 1% and 90% of tumor cells. Lewis- was also found in soluble form in sera from both normal donors and cancer patients using a newly developed enzyme-linked immunosorbent assay. Serum levels in patients was often less than 1á mg/mL, similar to no..
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Funding Acknowledgements
Supported by grants from The National Health and Medical Research Council of Australia (NHMRC). The Cancer Council of Australia, The Susan G. Komen Breast Cancer Foundation, The Bob Parker Memorial Trust and the Peter MacCallum Cancer Centre Foundation. Michael H. Kershaw and Phillip K. Darcy are supported by Career Development Awards from the National Breast Cancer Foundation and NHMRC. Mark J. Smyth and Joseph A. Trapani are Supported by NHMRC Senior Principal Research Fellowships. The authors also acknowledge the generous Support of C and M. Mori-is. Albert Park, Victoria, Australia for vector construction and production.