Narrowed TCR diversity for immunised mice challenged with recombinant influenza A-HIV Env(311-320) virus
Tania Cukalac, Jessica M Moffat, Vanessa Venturi, Miles P Davenport, Peter C Doherty, Stephen J Turner, John Stambas
VACCINE | ELSEVIER SCI LTD | Published : 2009
Understanding CD8+ T cell responses generated by live virus vectors is critical for the rational design of next generation HIV CTL-based vaccines. We used recombinant influenza viruses expressing the HIV Env(311-320) peptide in the neuraminidase stalk to study response magnitude, cytokine production and repertoire diversity for the elicited CD8+ D(d)Env(311) CTL set. The insertion of the CD8+ D(d)Env(311) epitope into the NA stalk resulted in a decrease in viral fitness that was reflected in lower lung viral titres. While not affecting the magnitude of endogenous primary influenza-specific responses, the introduction of the D(d)Env(311) CD8+ T cell epitope altered the hierarchy of responses ..View full abstract
Related Projects (1)
Awarded by NHMRC
Awarded by Australian Research Council Discovery
This work was supported by a NHMRC program grant (299907) awarded to PCD, a NHMRC Project grant awarded to JS (508902), an Australian Research Council Discovery Project DP0771340 (to MPD, SJT and VV), and the American Lebanese Syrian Associated Charities (ALSAC). The authors would like to thank Dr La Gruta and Dr Kedzierska for intellectual input and review of the manuscript.