Journal article

Narrowed TCR diversity for immunised mice challenged with recombinant influenza A-HIV Env(311-320) virus

Tania Cukalac, Jessica M Moffat, Vanessa Venturi, Miles P Davenport, Peter C Doherty, Stephen J Turner, John Stambas

VACCINE | ELSEVIER SCI LTD | Published : 2009

Abstract

Understanding CD8+ T cell responses generated by live virus vectors is critical for the rational design of next generation HIV CTL-based vaccines. We used recombinant influenza viruses expressing the HIV Env(311-320) peptide in the neuraminidase stalk to study response magnitude, cytokine production and repertoire diversity for the elicited CD8+ D(d)Env(311) CTL set. The insertion of the CD8+ D(d)Env(311) epitope into the NA stalk resulted in a decrease in viral fitness that was reflected in lower lung viral titres. While not affecting the magnitude of endogenous primary influenza-specific responses, the introduction of the D(d)Env(311) CD8+ T cell epitope altered the hierarchy of responses ..

View full abstract