Occurrence, Plasticity, and Evolution of the vpma Gene Family, a Genetic System Devoted to High-Frequency Surface Variation in Mycoplasma agalactiae
Laurent-Xavier Nouvel, Marc Marenda, Pascal Sirand-Pugnet, Eveline Sagne, Michelle Glew, Sophie Mangenot, Valerie Barbe, Aurelien Barre, Stephane Claverol, Christine Citti
JOURNAL OF BACTERIOLOGY | AMER SOC MICROBIOLOGY | Published : 2009
Mycoplasma agalactiae, an important pathogen of small ruminants, exhibits a very versatile surface architecture by switching multiple, related lipoproteins (Vpmas) on and off. In the type strain, PG2, Vpma phase variation is generated by a cluster of six vpma genes that undergo frequent DNA rearrangements via site-specific recombination. To further comprehend the degree of diversity that can be generated at the M. agalactiae surface, the vpma gene repertoire of a field strain, 5632, was analyzed and shown to contain an extended repertoire of 23 vpma genes distributed between two loci located 250 kbp apart. Loci I and II include 16 and 7 vpma genes, respectively, with all vpma genes of locus ..View full abstract
This work was supported by the French Ministry of Agriculture and Fisheries and by the National Institute for Agricultural Research (INRA).