Journal article
Small-molecule induction of neural crest-like cells derived from human neural progenitors
R Hotta, L Pepdjonovic, RB Anderson, D Zhang, AJ Bergner, J Leung, A Pébay, HM Young, DF Newgreen, M Dottori
Stem Cells | WILEY | Published : 2009
DOI: 10.1002/stem.208
Abstract
Neural crest (NC) cells are stem cells that are specified within the embryonic neuroectodermal epithelium and migrate to stereotyped peripheral sites for differentiation into many cell types. Several neurocristopathies involve a deficit of NC-derived cells, raising the possibility of stem cell therapy. In Hirschsprung's disease the distal bowel lacks an enteric nervous system caused by a failure of colonization by NC-derived cells. We have developed a robust method of producing migrating NC-like cells from human embryonic stem cell-derived neural progenitors using a coculture system of mouse embryonic fibroblasts. Significantly, subsequent exposure to Y27632, a small-molecule inhibitor of th..
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Funding Acknowledgements
We thank Craig Smith, Murdoch Children's Research Institute, Melbourne, for SoxE antibody, and Miles Epstein, University of Wisconsin, for the human anti-Hu. This study was supported by The University of Melbourne, The National Health and Medical Research Council of Australia, and Friedreich Ataxia Research Association Australia.