Facile Pretreatment of Bacilius circulans beta-Galactosidase Increases the Yield of Galactosyl Oligosaccharides in Milk and Lactose Reaction Systems
Aaron Gosling, Johan Alftren, Geoff W Stevens, Andrew R Barber, Sandra E Kentish, Sally L Gras
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | AMER CHEMICAL SOC | Published : 2009
The commercially available preparation of beta-galactosidase from Bacillus circulans , known as Biolacta FN5, has been extensively used in the production of prebiotic galactooligosaccharides (GOS). This study focuses on characterizing the production of GOS in two reaction systems: 10% lactose (w/v) in buffer and skim milk. Analysis of the temperature dependence of the GOS yield along with the relative rates of GOS synthesis and degradation leads to the finding that GOS degradation activity was selectively decreased in Biolacta FN5 above 40 degrees C. Facile heat treatment of Biolacta FN5 solution prior to use allowed for GOS yields to be significantly increased in both reaction systems.
We acknowledge Daniel Otieno for preliminary work leading to this study and Chiranjib Bhattacharjee from Jadavpur University, Kolkata, India, and P. K. Bhattacharya from the Indian Institute of Technology, Kanpur, India, for useful discussions. We also acknowledge the Particulate Fluids Processing Centre, Special Research Centre of the Australian Research Council (ARC), for their support.