Integrated genome-wide DMA copy number and expression analysis identifies distinct mechanisms of primary chemoresistance in ovarian carcinomas

Abstract

Purpose; A significant number of women with serous ovarian cancer are intrinsically refractory to platinum-based treatment. We analyzed somatic DIM A copy number variation and gene expression data to identify key mechanisms associated with primary resistance in advanced- stage serous cancers. Experimental Design: Genome-wide copy number variation was measured in 118 ovarian tumors using high-resolution oligonucleotide microarrays. A well-defined subset of 85 advanced-stage serous tumors was then used to relate copy number variation to primary resistance to treatment. The discovery-based approach was complemented by quantitative-PCR copy number analysis of 12 candidate genes as independent va..