AMPK activation is fiber type specific in human skeletal muscle: effects of exercise and short-term exercise training
Robert S Lee-Young, Benedict J Canny, Damian E Myers, Glenn K McConell
JOURNAL OF APPLIED PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2009
AMP-activated protein kinase (AMPK) has been extensively studied in whole muscle biopsy samples of humans, yet the fiber type-specific expression and/or activation of AMPK is unknown. We examined basal and exercise AMPK-alpha Thr(172) phosphorylation and AMPK subunit expression (alpha(1), alpha(2), and gamma(3)) in type I, IIa, and IIx fibers of human skeletal muscle before and after 10 days of exercise training. Before training basal AMPK phosphorylation was greatest in type IIa fibers (P < 0.05 vs. type I and IIx), while an acute bout of exercise increased AMPK phosphorylation in all fibers (P type IIa > type I), irrespective of training status. Thus skeletal muscle AMPK phosphorylation a..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
This work was supported by a grant from the National Health and Medical Research Council (NHMRC) of Australia (no. 237002) and Diabetes Australia (G. K. McConell).