Journal article
Examination of 'lipotoxicity' in skeletal muscle of high-fat fed and ob/ ob mice
SM Turpin, JG Ryall, R Southgate, I Darby, AL Hevener, MA Febbraio, BE Kemp, GS Lynch, MJ Watt
Journal of Physiology | WILEY | Published : 2009
Abstract
Excess lipid accumulation resulting from an elevated supply of plasma fatty acids is linked to the pathogenesis of the metabolic syndrome and heart disease. The term 'lipotoxicity' was coined to describe how lipid accumulation leads to cellular dysfunction and death in non-adipose tissues including the heart, pancreas and liver. While lipotoxicity has been shown in cultured skeletal muscle cells, the degree of lipotoxicity in vivo and the functional consequences are unresolved. We studied three models of fatty acid overload in male mice: 5 h Intralipid® and heparin infusion, prolonged high fat feeding (HFF) and genetic obesity induced by leptin deficiency (ob/ob mice). Markers of apoptosis, ..
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Awarded by National Institute of Diabetes and Digestive and Kidney Diseases
Funding Acknowledgements
We thank Drs Rudolf Zechner and Gunter Haemmerle (University of Graz) for providing the ATGL null mice and Ronnie Minnard for expert technical advice. These studies were supported by research grants from the Australian Research Council (ARC) and the National Health and Medical Research Council (NHMRC) of Australia. S.M.T. is supported by a Dora Lush Scholarship, J.G.R. by a C. J. Martin Postdoctoral Fellowship, M.A.F. by a Principal Research Fellowship and M.J.W. by a R. Douglas Wright Career Development Award from the NHMRC. R.S. is supported by an Australian Postdoctoral Fellowship and B.E.K. a Federation Fellowship from the ARC. A.L.H. is supported by the National Institutes of Health (DK073227).