Journal article

Endothelial Precursor Cells Home to a Vascularized Tissue Engineering Chamber by Application of the Angiogenic Chemokine CXCL12

Jeremy W Simcock, Anthony J Penington, Wayne A Morrison, Erik W Thompson, Geraldine M Mitchell



Tissue engineering of vascularized constructs has great utility in reconstructive surgery. While we have been successful in generating vascularized granulation-like tissue and adipose tissue in an in vivo tissue engineering chamber, production of other differentiated tissues in a stable construct remains a challenge. One approach is to utilize potent differentiation factors, which can influence the base tissue. Endothelial precursor cells (EPCs) have the ability to both carry differentiation factors and home to developing vasculature. In this study, proof-of-principle experiments demonstrate that such cells can be recruited from the circulation into an in vivo tissue engineering chamber. CXC..

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Funding Acknowledgements

The authors wish to acknowledge EMSU staff, St. Vincent's Hospital-Sue Mc Kay, Liliana Pepe, Anna Deftereos, and Amanda Rixon-for assistance in animal surgery, Jason Palmer for assistance in histology, Effie Keramidaris for assistance in EPC preparation, and Tony Blicks for assistance with RT-PCR. The EPCs were kindly donated by the laboratory of Prof. Silviu Itescu, University of Melbourne, Department of Medicine, St. Vincent's Hospital; J. Simcock was the recipient of a University of Melbourne Scholarship and a Royal Australasian College of Surgeons Scholarship. The project was also supported by a St. Vincent's Hospital Research Grant.