Sexually Dimorphic MicroRNA Expression During Chicken Embryonic Gonadal Development
Stephanie C Bannister, Mark LV Tizard, Timothy J Doran, Andrew H Sinclair, Craig A Smith
BIOLOGY OF REPRODUCTION | OXFORD UNIV PRESS INC | Published : 2009
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs in gonadal development and sex differentiation has not yet been reported. Using the chicken embryo as a model, microarrays were used to profile the expression of chicken miRNAs prior to, during, and after the time of gonadal sex differentiation (Embryonic Day 5.5 [E5.5], E6.5, and E9.5). Sexually dimorphic miRNAs were identified, and the expression patterns of several were subjected to further validatio..View full abstract
Awarded by National Center for Biotechnology Information Gene Expression Omnibus
Supported by funding from CSIRO Emerging Sciences Initiative (M.L.V.T.), and the Australian Research Council Discovery Grant to C. A. S. and A. H. S. Microarray data were deposited in the National Center for Biotechnology Information Gene Expression Omnibus (GEO) database under the accession number GSE13154.