Effect of local, long-term delivery of platelet-derived growth factor (PDGF) on injected fat graft survival in severe combined immunodeficient (SCID) mice
Randall O Craft, John Rophael, Wayne A Morrison, Aditya V Vashi, Geraldine M Mitchell, Anthony J Penington
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY | ELSEVIER SCI LTD | Published : 2009
SUMMARY BACKGROUND: Autogenous fat injection is widely used for the correction of acquired and congenital soft tissue defects. However, the high absorption rate results in the need for over-correction of the defect and repeat procedures. We hypothesised that platelet-derived growth factor (PDGF), a potent mitogen and known stimulant for murine preadipocytes, would improve fat graft survival when concentrations were sustained with a gelatine microsphere delivery system. METHODS: Abdominal fat was harvested from an otherwise healthy 43-year-old woman during a breast reconstruction. Prior to subdermal injection into severe combined immunodeficient (SCID) mice, the fat grafts were divided into 1..View full abstract
Dr. Randall Craft was funded by the Mayo Foundation. The authors thank Sue McKay, Litiana Pepe, and Anna Defteros for their surgical assistance.