Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach
Mitra Sharbatkhori, Hossein Mirhendi, Aaron R Jex, Aradhana Pangasa, Bronwyn E Campbell, Eshrat B Kia, Mohammad R Eshraghian, Majid F Harandi, Robin B Gasser
ELECTROPHORESIS | WILEY | Published : 2009
In the present study, we have extended earlier taxonomic, biochemical and experimental investigations to characterize Echinococcus granulosus from various hosts in Iran utilizing DNA regions (designated pcox1 and pnad1) within the cytochrome c oxidase subunit 1 and NADH dehydrogenase 1 mitochondrial genes, respectively. An emphasis was placed on the characterization of E. granulosus isolates (cyst material) from humans, sheep, goats, cattle and camels, and on assessing their genetic relationships. PCR-based SSCP analysis of pcox1 and pnad1 amplicons derived from individual isolates (n=148) of E. granulosus revealed five (pc1-pc5) and nine (pn1-pn9) electrophoretic profiles, respectively. Seq..View full abstract
Awarded by Tehran University of Medical Sciences
Awarded by Ministry of Health of the Iranian Government
This project was funded in part by the Tehran University of Medical Sciences (project no. 85-04-27-4766) and The University of Melbourne (RBG). MS was the recipient of a scholarship (no. 46755) from the Ministry of Health of the Iranian Government. Thanks are due to Min Hu, Nathan Bott, Matthew Nolan and Cinzia Cantacessi for discussions and support during the course of this study, Antti Lavikainen for constructive comments on the manuscript and H.R. Rahimi, F. Zahabiun and N. Jalalizandfor assistance. RBGs laboratory is currently in receipt of grants from the Australian Research Council (ARC), Melbourne Water Corporation, and Meat and Livestock Australia (MLA).