Journal article
Bioinformatic analysis of abundant, gender-enriched transcripts of adult Ascaris suum (Nematoda) using a semi-automated workflow platform
C Cantacessi, FC Zou, RS Hall, W Zhong, AR Jex, BE Campbell, S Ranganathan, PW Sternberg, XQ Zhu, RB Gasser
Molecular and Cellular Probes | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2009
Abstract
Expressed sequence tag (EST) data representing transcripts with a high level of differential hybridization in suppressive-subtractive hybridization (SSH)-based microarray analysis between adult female and male Ascaris suum were subjected to detailed bioinformatic analysis. A total of 361 ESTs clustered into 209 sequences, of which 52 and 157 represented transcripts that were enriched in female and male A. suum, respectively. Thirty (57.7%) of the 'female' subset of 52 sequences had orthologues/homologues in other parasitic nematodes and/or Caenorhabditis elegans, 13 (25%) exclusively in other parasitic nematodes and nine (17.3%) had no match in any other organism for which sequence data are ..
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Awarded by Howard Hughes Medical Institute
Funding Acknowledgements
This work was supported by the Australian Research Council, the Australian Academy of Science and the Australian-American Fulbright Commission (REG), the Howard Hughes Medical Institute and WormBase (PWS), the China National Science Funds for Distinguished Young Scientists (grant no. 30225033), the National Natural Science Foundation of China (grant no. 30671578), and the Natural Science Foundation of Guangdong Province (key project, grant no. 36835) (XQZ). CC is the grateful recipient of International Postgraduate Research Scholarship (IPRS) from the Australian Government and a fee-remission scholarship through the University of Melbourne, and the Sir Ian Clunies Ross Prize (2008) and Dr Sue Newton (2009) Award from the Faculty of Veterinary Science, The University of Melbourne.