A combined microscopic-molecular method for the diagnosis of strongylid infections in sheep
Nathan J Bott, Bronwyn E Campbell, Ian Beveridge, Neil B Chilton, Dianne Rees, Peter W Hunt, Robin B Gasser
INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2009
We evaluated a combined microscopic-molecular approach for the diagnosis of key strongylid infections in sheep using panels of well-defined control and test samples. The method established is based on the separation of nematode eggs from faecal samples using a salt flotation procedure, the extraction and column-purification of genomic DNA, followed by real-time PCR and melting-curve analysis. Specific and semi-quantitative amplification from (a minimum of 0.1-2.0pg) genomic DNA of Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus spp., Cooperia oncophora, Oesophagostomum columbianum, Oesophagostomum venulosum or Chabertia ovina is achieved using a specific, forward oligonucle..View full abstract
Funding from Meat and Livestock Australia (MLA) and the Australian Research Council (ARC) is acknowledged. Thanks go to David Callan for providing some samples, Garry Anderson for assistance with statistical analyses, and Paul Monis, John Larsen, Angus Campbell and Norman Anderson for discussions and support. Thanks to other colleagues, particularly Herve Hoste, for providing some samples.