Journal article
Inducer-dependent nuclear localization of a zn(ii)2cys6 transcriptional activator, amyr, in aspergillus nidulans
T Makita, Y Katsuyama, S Tani, H Suzuki, N Kato, RB Todd, MJ Hynes, N Tsukagoshi, M Kato, T Kobayashi
Bioscience Biotechnology and Biochemistry | TAYLOR & FRANCIS LTD | Published : 2009
DOI: 10.1271/bbb.80654
Abstract
AmyR is a Zn(II)2Cys6 transcriptional activator that regulates expression of the amylolytic genes in Aspergillus species. Subcellular localization studies of GFP-fused AmyR in A. nidulans revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physiological inducer of the amylolytic genes. The C-terminal domains of AmyR, designated MH3 (residues 419-496) and MH4 (residues 516-542), were essential for sensing the inducing stimulus and regulating the subcellular localization. The MH2 domain (residues 234-375) located in the middle of AmyR was required for transcriptional activation of the target genes, and the nuclear localization signals were id..
View full abstractGrants
Funding Acknowledgements
The authors would like to thank Dr. Y. Niwa for generously providing the sGFP gene. This work was partially supported by Grants-in-Aid for Scientific Research (B) and (C) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to T.K.