Journal article

Translation-Linked mRNA Destabilization Accompanying Serum-Induced Nox4 Expression in Human Endothelial Cells

Hitesh Peshavariya, Fan Jiang, Caroline J Taylor, Stavros Selemidis, Catherine WT Chang, Gregory J Dusting

ANTIOXIDANTS & REDOX SIGNALING | MARY ANN LIEBERT, INC | Published : 2009

Abstract

NADPH oxidase is involved in cell signaling, regulating proliferation of vascular cells, especially in endothelium. The Nox4 catalytic subunit has a major role in endothelial cells, but growth arrest of cultured endothelial cells following serum deprivation paradoxically increases mRNA for Nox4. We investigated the relationships between Nox4 mRNA stability and protein expression in human microvascular endothelial cells. Serum starvation increased the steady-state level of Nox4 mRNA but paradoxically diminished Nox4 protein expression. mRNA transcription in the absence of serum is maintained by the p38MAP kinase pathway, for inhibition of p38MAP kinase reduced both Nox4 mRNA and Nox4 promoter..

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Grants

Awarded by NHMRC


Awarded by Dora Lush scholarship


Funding Acknowledgements

Dr. Hitesh Peshavariya was supported by NHMRC project grant ( ID 350482) and Dora Lush scholarship ( ID 327401). Prof. Greg Dusting is a Principal Research Fellow of NHMRC ( ID 400303). The adenovirus construct of Nox4 and dominant negative Nox4 was donated by Kalyankar Mahadev and Barry J. Goldstein, Division of Endocrinology, Diabetes, and Metabolic Diseases, Department of Medicine, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania, USA.