Journal article
3′,4′-Dihydroxyflavonol down-regulates monocyte chemoattractant protein-1 in smooth muscle: Role of focal adhesion kinase and PDGF receptor signalling
F Jiang, N Guo, GJ Dusting
British Journal of Pharmacology | Published : 2009
Abstract
Background and purpose: We investigated the effects of a synthetic flavonol, 3′,4′-dihydroxyflavonol (DiOHF) on the expression of monocyte chemoattractant protein-1 (MCP-1) in rat vascular smooth muscle cells. Experimental approach: MCP-1 expression was assessed by quantitative real-time PCR and protein phosphorylation by immunoprecipitation and Western blots. Key results: DiOHF (1-30 μmol-L -1) concentration-dependently reduced MCP-1 expression in both quiescent cells and cells stimulated with platelet-derived growth factor (PDGF) or interleukin 1-β. The effect of DiOHF was associated with a suppression of focal adhesion kinase (FAK)-mediated signalling. In vitro kinase assays demonstrated ..
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Funding Acknowledgements
The authors thank Dr K.M. Yamada (National Institutes of Health, Bethesda, MD, USA) for providing us the pRK-VSV-FRNK plasmids. This work was supported by Project Grants from the National Health and Medical Research Council of Australia and Grants-in-Aid from the National Heart Foundation of Australia. G.J.D. receives an National Health and Medical Research Council Principal Research Fellowship.