Journal article

Rapid evolution of mouse Y centromere repeat DNA belies recent sequence stability

Mark D Pertile, Alison N Graham, KH Andy Choo, Paul Kalitsis



The Y centromere sequence of house mouse, Mus musculus, remains unknown despite our otherwise significant knowledge of the genome sequence of this important mammalian model organism. Here, we report the complete molecular characterization of the C57BL/6J chromosome Y centromere, which comprises a highly diverged minor satellite-like sequence (designated Ymin) with higher-order repeat (HOR) sequence organization previously undescribed at mouse centromeres. The Ymin array is approximately 90 kb in length and resides within a single BAC clone that provides sequence information spanning an endogenous animal centromere for the first time. By exploiting direct patrilineal inheritance of the Y chro..

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Awarded by Mouse Chromosome Y Mapping Project

Awarded by National Health and Medical Research Council of Australia (NHMRC)

Funding Acknowledgements

We thank Emma Northrop for technical advice with ChIP, and John Mitchell and Charles Robin for helpful discussions. The sequence and positional information of BAC done RP24-110P17 was established by the Mouse Chromosome Y Mapping Project (Jessica E. Alfoldi, Helen Skaletsky, Steve Rozen, and David C. Page) at the Whitehead Institute for Biomedical Research, Cambridge, MA in collaboration with the Washington University Genome Sequencing Center, St. Louis, MO. Research in our laboratory has been supported by the National Health and Medical Research Council of Australia (NHMRC). P. K. is an RD Wright Fellow and K. H. A. C. is a Senior Principal Research Fellow of the NHMRC. M. D. P. is the recipient of NHMRC Scholarship ID 437024.