Activation of a prometastatic gene expression program in hypoxic neuroblastoma cells
Preamrudee Poomthavorn, Sheena HX Wong, Sandra Higgins, George A Werther, Vincenzo C Russo
ENDOCRINE-RELATED CANCER | BIOSCIENTIFICA LTD | Published : 2009
The hypoxia inducible factor-1alpha (HIF1alpha) is a key regulator of oxygen homeostasis, modulating cell survival, and growth in cells exposed to hypoxia. In this study, neuroblastoma (NB) cells SH-SY5Y and SK-N-MC were employed to determine the mechanisms regulating adaptation to hypoxia. NB cells were cultured in a serum-free medium in the presence or absence of CoCl(2) (100 muM, hypoxia mimic) for up to 48 h. SH-SY5Y and SK-N-MC cell numbers were not affected by CoCl(2) treatment, while mitochondrial activity was reduced by approximately 50% in SH-SY5Y cells and by approximately 70% in SK-N-MC cells. Intracellular accumulation of HIF1alpha protein was detected as early as 30 min of post-..View full abstract
Awarded by National Health and Medical Research Council of Australia (NHMRC)
This work was supported by the National Health and Medical Research Council of Australia (NHMRC Project Grant No-436955 to V C R, GAW from 2007 to 2009).