Journal article

Molecular analysis of the PArkin co-regulated gene and association with male infertility

Gabrielle R Wilson, Marcus L-J Sim, Kate M Brody, Juliet M Taylor, Robert I McLachlan, Moira K O'Bryan, Martin B Delatycki, Paul J Lockhart



OBJECTIVE: To investigate the potential role of PArkin co-regulated gene (PACRG) in human male infertility. DESIGN: Case-control study. SETTING: Academic reproductive biology department. PATIENT(S): Blood samples were obtained from 610 patients and 156 normal control subjects. INTERVENTION(S): Genomic DNA was used as template for polymerase chain reaction amplification of the PACRG promoter and coding exons. The amplified fragments were tested for DNA sequence variations by direct sequencing and restriction enzyme analysis. MAIN OUTCOME MEASURE(S): Gene structure and sequence alterations of PACRG in infertile male patients. RESULT(S): The structure of PACRG was determined to comprise 5 codin..

View full abstract


Awarded by National (Australia) Health and Medical Research Council (NHMRC)

Funding Acknowledgements

Supported in part by National (Australia) Health and Medical Research Council (NHMRC) project grant no. 436977 to P.J.L. and by funding from Andrology Australia and Monash IVF, Pty., Ltd. G.R.W. holds an NHMRC Postgraduate Scholarship (no. 384489), M.K.O.B. is an NHMRC Senior Research Fellow (no. 384132), R.I.McL. is an NHMRC Principal Research Fellow (no. 441103), M.B.D. is an NHMRC Practitioner Fellow (no. 284520), and P.J.L. is an NHMRC R.D. Wright Fellow (no. 334346).