Disruption of Mitochondrial Malate-Aspartate Shuttle Activity in Mouse Blastocysts Impairs Viability and Fetal Growth
Megan Mitchell, Kara S Cashman, David K Gardner, Jeremy G Thompson, Michelle Lane
BIOLOGY OF REPRODUCTION | SOC STUDY REPRODUCTION | Published : 2009
The nutrient requirements and metabolic pathways used by the developing embryo transition from predominantly pyruvate during early cleavage stages to glucose at the blastocyst; however, the complexities involved in the regulation of metabolism at different developmental stages are not clear. The aims of this study were to examine the role of the malate-aspartate shuttle (MAS) in nutrient metabolism pathways in the developing mouse blastocyst and the consequences of impaired metabolism on embryo viability and fetal and placental growth. Eight-cell-stage mouse embryos were cultured in the presence of the MAS inhibitor amino-oxyacetate, with or without pyruvate as an energy substrate in the med..View full abstract
Supported by an Australian National Health and Medical Research Council (NHMRC) Project Grant and an NHMRC Program Grant. J.G.T is a recipient of an NHMRC Senior Research Fellowship, and M. L. is a recipient of an R. D. Wright Fellowship from the NHMRC.